VitroGel® RGD Kit

VitroGel® RGD High Concentration Tunable, xeno-free hydrogel with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. Room Temperature Operation Room temperature protocol/operation. No ice bucket required. Xeno-free 100% synthetic. Animal & human origin-free, biofunctional hydrogel. Tunable Hydrogel Strength Adjust the hydrogel strength from 10 Pa to over 20,000 Pa to create the optimal cell environment. Mix & Match Build and create a customized multifunctional hydrogel by blending different types of VitroGel® together. Easy-to-use No cross-linking agent required. Adjust hydrogel with Dilution Solution, mix with cells, add medium and incubate. Easy cell harvesting Simple and efficient cell harvesting by the non-enzymatic VitroGel® Organoid Recovery Solution. VitroGel® RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel® RGD High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.  VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Organoid Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies. From 3D cell culture to 2D cell coating and animal injection, VitroGel® enables the seamless integration of in vitro and in vivo studies using the same platform system. Mix & Match – 3D Cell Culture Your WAY! Unique to VitroGel® High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel.  VitroGel® RGD High Concentration can be “mix & matched” with other VitroGel High Concentration hydrogels such as VitroGel® IKVAV, VitroGel® YIGSR, VitroGel® MMP, and VitroGel® COL to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture microenvironment for various applications. Learn more about the “Mix & Match” features > Specifications ContentsVitroGel® RGD High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL Hydrogel FormulationXeno-free tunable hydrogel modified with RGD peptide. UseGood for adhesion cells or cells requiring stronger cell-matrix interactions. Mix & MatchCan be blended with other versions of VitroGel® concentrated hydrogels to create a custom multi-functional matrix. OperationRoom temperature Hydrogel Strength10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. pHNeutral ColorTransparent Cell HarvestingVitroGel® Organoid Recovery Solution5-15 min cell recovery InjectableInjectable hydrogel StorageStore at 2-8°C. Ships at ambient temperature Number of UsesDilution ratio: 1:2 = 225 uses at 50 µL per well1:3 = 300 uses at 50 µL per well1:5 = 450 uses at 50 µL per well 3D Cell Culture Process in 20 Minutes VitroGel® High Concentration hydrogels are easy to use. There is no cross-linking agent required. Work confidently at room temperature. Tunable Hydrogel Strength Simply diluting the hydrogel controls the gel strength. Protocols and Resources VitroGel® RGD Protocol Xenograft/Injection Protocol Five Cell Culture Methods for VitroGel® Protocol Cell Recovery/Harvesting Protocol Live-Dead Cell Viability Assay Protocol Cell Fluorescent Staining in Gel for Nuclei and Actin Filament Protocol RNA/DNA Extraction, Cell Lysis for Downstream Analysis Protocol Cell Proliferation Assay Protocol Immunofluorescence Staining Protocol Sample Preparation for Histological Analysis Protocol Product Documentation Sales Sheet – VitroGel® High Concentration Product Data Sheet Material Safety Data Sheet (MSDS) Frequently Asked Questions  Video Protocols & Demonstrations VIDEO PROTOCOL TIP 3D Cell Culture VitroGel High Concentration Hydrogels VIDEO PROTOCOL TIP 2D Cell Coating Method 1 VitroGel High Concentration Hydrogels VIDEO PROTOCOL TIP 2D Cell Coating Method 2 VitroGel High Concentration Hydrogels VIDEO PROTOCOL TIP Cell Recovery Method 1 from 3D & 2D cultures VIDEO PROTOCOL TIP Cell Recovery Method 2 from 3D & 2D  cultures VIDEO PROTOCOL TIP Injectable Hydrogel VitroGel System Webinars Ex Vivo Model Lymphomoids: A Tissue-based Ex Vivo Culture System for Lymphoma Therapy Screening Ex Vivo Model A Novel Pre-Clinical Model of the Normal Human Breast Research Highlights Research Highlights Unlocking Gastric Cancer’s Immune Evasion Secrets with a Key Enzyme and Xeno-Free VitroGel® Research Highlights Prolonged Dexamethasone Delivery Using VitroGel® for Protection Against Cisplatin-Induced Ototoxicity Research Highlights Game Changer for Lymphoma Therapy Screening Research Highlights VitroGel®-Enhanced 3D Culture Models Reveal the Potential of Basroparib in Overcoming MEK Inhibitor Resistance in KRAS-Mutated Colorectal Cancer Research Highlights An Artificial Womb for the Womb Application Notes Application Notes 3D Invasion of Glioblastoma Cells in VitroGel® Hydrogel System Application Notes Long-Term Neuron Culture Maturation in 3D Hydrogel Constructs Application Notes 3D Cell Culture of Human Colon Cancer Cells (HCT116) on VitroGel® System Application Notes 3D Cell Culture of Human Pancreatic Cancer Cells (PANC-1) on VitroGel® System Application Notes Long Term 3D Tumor Spheroid Culture in VitroGel® Hydrogel Matrix Application Notes Advanced Skin Cell Models Using the VitroGel® System: 2D Coating and 3D Cell Culture with Human Dermal Fibroblasts Data and References Cell Type Behavior Reference Table – VitroGel® RGD Multiple studies have made use of RGD hydrogel in different tissues and cell types. RGD is commonly used as an immobilized, adhesive ligand in 3D hydrogels, allowing researchers to study many different cellular processes and behaviors in normal physiological and pathological contexts. Bone Cell TypeBehavior Goat bone marrow stromal cellsPromoted osteogenic differentiation Rat bone marrow stromal cellsPromoted osteogenic differentiation Rat osteoblastsIncreased cell attachment and spreading Cancer/Tumor Cell TypeBehavior Biphasic synovial sarcoma SYO-1Cell proliferation and cell matrix interactions Bone OSA 1777Cell proliferation and cell matrix interactions Breast 4T1 Cell proliferation, division, migration, and invasion Breast AU-565Cell proliferation and cell matrix interactions Breast Cancer MCF-7Cell proliferation, intercellular connections Breast E0771Cell proliferation and cell matrix interactions Breast MDA-MB-231Cell proliferation, division, migration, and invasion Breast T47DCell proliferation, division, migration, and invasion Colorectal adenocarcinoma DLD-1 cellsCell proliferation and cell matrix interactions Epithelial ovarian OV-MZ-6Promoted spheroid formation and proliferation Epithelial ovarian SKOV-3Promoted spheroid formation and proliferation Fuji CellsCell proliferation and cell matrix interactions Glioblastoma SF 268Cell proliferation and cell matirx interaction Glioblastoma SF 295Cell proliferation and cell matirx interaction Glioblastoma SNB75Cell proliferation and cell matirx interaction Glioblastoma U-251 MGCell proliferation and cell matirx interaction Glioma U87-MGIncreased cell spreading and actin stress fiber assembly Glioma U87-MGCell proliferation and cell matirx interaction Glioma U373-MGIncreased cell adhesion duration and migration (on higher stiffness) HEK 293Cell proliferation and cell matrix interactions Huaman colon carcinoma HCT-8Cell proliferation and cell matirx interaction Human colorectal carcinoma HCT 116cell proliferation, cell survival, and intercelluar networking Human pancreatic cancer PANC-1cell proliferation and cellular interactions Insulinoma ins-1 (Rat)Cell proliferation and cell matrix interactions Liver carcinoma HepG2Cell proliferation and cell matirx interaction Melanoma CellsCell proliferation and cell matrix interactions Ovarian carcinoma OVCAR-3Cell proliferation and invasion Primary glioblastom U87cell proliferation and cellular interactions Prostate adenocarcinoma LNCaPIncreased cell attachment Prostate CRPCCell proliferatin and invasion Prostate DU145Cell proliferation and invasion Prostate PC3Cell proliferation and invasion Cartilage Cell TypeBehavior Bovine chondrocytesIncreased cell viability and proliferation Bovine chondrocytesPromoted cell attachment, viability, and stress fiber formation Human chondrocytesPromoted cell viability and proliferation Connect Tissues Cell TypeBehavior Fibroblast NIH3T3 Promoted cell spreading Fibroblasts NIH3T3Increased directional cell migration toward gradient Human dermal fibroblasts Promoted cell survival and spreading Human dermal fibroblastsIncreased cell adhesion and proliferation Human foreskin fibroblastsPromoted cell spreading Epithelial Cells Cell TypeBehavior A549 cellsCell proliferation and invasion MCF-12ACell proliferation and invasion Mouse ovarian follicle cellsCell proliferation and invasion Immune Cells Cell TypeBehavior Beta TC3 CellsCell proliferation and cellular interactions Kidney Cell TypeBehavior Human embryonic kidney HEK293Promoted spheroid formation Madin-Darby Canine KidneyPromoted formation of structured epithelial cysts Liver Cell TypeBehavior Human hepatocytesIncreased number of filopodia and synthesis of albumin Mouse hepatocytesPromoted cell viability Lung Cell TypeBehavior Alveolar epithelial A549Inhibited cell detachment Alveolar epithelial RLE-6TNIncreased cell attachment and mesenchymal differentiation Muscle Cell TypeBehavior Mouse skeletal myoblastsPromoted cell attachment, proliferation, and myofibril formation Myoblasts C2C12Promoted cell proliferation and differentiation Neural Cell TypeBehavior Chick dorsal root ganglion cellsIncreased neurite length, neurite outgrowth, and neurite number In vivo lesioned rat cortexSupported angiogenesis and inhibited glial scars In vivo lesioned rat spinal cordSupported angiogenesis and axon regeneration Stem Cells Cell TypeBehavior Human embryonic stem cellsIncreased retinal pigmented epithelium and optic vesicle development Human iPSCCell proliferation, and cell matrix interactions Human mesenchymal stem cellsIncreased cell viability Mouse embryonic stem cells Promoted endothelial cell differentiation Mouse mesenchymal stem cellsPromoted cell spreading and migration Rat mesenchymal stem cellsIncreased cell adhesion and spreading Rat mesenchymal stem cellsPromoted cell attachment and differentiation Vascular/Cardiac Cell TypeBehavior Human aortic smooth muscle cellsPromoted cell attachment Human umbilical vein endothelial cellsIncreased cell adhesion, proliferation, migration, and angiogenesis Human umbilical vein endothelial cellsIncreased cell adhesion and proliferation Rat neonatal cardiacPromoted cell attachment and tissue regeneration and prevented apoptosis View All Cell Type Behaviors for All VitroGel Products TISSUE/ORGAN TYPECELL TYPEREADY TO USE HIGH CONCENTRATIONBEHAVIOR Beta Cell BL5 human beta cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3D, VitroGel MMPEnhance spheroids formation Beta TC3 cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cellular interactions Bone Bone marrow stromal cells (rat)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMPOsteogensic differentiation , Cell attachment and osteoblast differentiation,Cell proliferation, cell viability, and cellular networking. Osteoblasts (rat) VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell attachment and spreading Bone marrow stromal cells (bovine) VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell spreading and osteocalcin expression BreastMammary gland MCF10AVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitrolGel RGD, VitroGel COL, VitroGel MMPSpheroid formation, MMP activity in response to TGF-B1 Mammary epithelium (mouse)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell invasion and dissemination Cancer/TumorHuman colorectal carcinoma HCT 116VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation, cell survival, and intercellular networking Huaman colon carcinoma HCT-8 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Glioma U87-MG VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel MMP, VitroGel COLCell spreading and acting stress fiber assembly, cell proliferation, spreading, and migration, Cell migration dependent on mechancial force, Cell proliferation and cell matrix interaction Gliobastoma SF 268VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitrolGel RGDCell proliferation and cell matrix interaction Gliobastoma SF 295VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Glioblastoma SNB75VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Glioblastoma U-251 MGVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Prostate PC3VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel COL, VitroGel IKVAV, VitroGel RGD, VitroGel MMPCell proliferation, reduced MMP release, invasion, migration, and spheroid metabolic activity. Prostate LNCaPVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell attachment, proliferation, and prostate specific antigen release Prostate CRPCVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and invasion Prostate DU145VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and invasion Melanoma B16F10VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel COL, VitroGel YIGSRCell migration, invasion, MMP release, cell attachment and spreading Breast MDA-MB-231VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel MMP, VitroGel 3DCell invasion, spreading, proliferation, division, migration, and cluster growth Fibrosarcoma HT1080VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell infiltration, attachment Breast T47DVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel COL, VitroGel 3D, VitroGel RGD, VitroGel MMPForce dependent tubule formation, cell cluster growth, spheroid formation and proliferation Breast 4T1VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation Breast CTCVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3D, VitroGel RGDCell proliferation Breast E0771VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation spheroid formation Brest AU-565VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation cell matrix interactions Epithelial ovarian OV-MZ-6 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDSpheroid formation and proliferation Epithelial ovarian SKOV-3 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDSpheroid formation and proliferation Glioma U373-MG VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMPCell adhesion, invasion and migration Rhabdomyosarcoma (human) VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel YIGSRCell attachment and spreading Melanoma SK-MEL-28 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel IKVAVCell adhesion and proliferation Melanoma K-1735VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel IKVAVCell invasion Melanoma A2058VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel IKVAVCollagenolytic activity Brainstem glioma DIPGVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMPCell proliferation and survival Hela Cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3D, VitroGel RGD, VitroGel MMPCell proliferation Colorectal adenocarcinoma DLD-1 cellsVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Giloma LRM55VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel IKVAV, VitroGel MMPCell attachment Melanoma WM 239AVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMPCell invasion Melanoma Cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Insulinoma ins-1 (Rat)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Biphasic synovial sarcoma SYO-1VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation, cell matrix interation, and cell survival Fuji Cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and cell matrix interaction Chordoma Cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DCell proliferation Bone OSA 1777VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDSpheroid and cluster formation Glioma RuGliVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel COL Integrin dependent cell adhesion Breast Cancer MCF-7 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMP, VitroGel 3DCell proliferation, intercellular connections, morphological changes, MMP expression, and angiogenesis Liver carcinoma HepG2 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell viability, growth, drug resistance, proliferation, and cellular matrix interaction Human pancreatic cancer PANC-1 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMPCell proliferation and cellular interactions Primary breast (human)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMPCell invasion, migration, and dissemination Ovarian carcinoma OVCAR-3 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel MMPCell proliferation, cell matrix interactions Ovarian OVCA429 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel MMP, VitroGel COLMMP dependent cell invasion Human Osteosarcoma KHOSVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel 3DCell proliferation and spheroids formation Human Osteosarcoma U2OSVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel 3DCell proliferation and spheroids formation Human fibroblast-like synoviocytes (FLS)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DCell proliferation and inflammatory responses Human Liposarcoma 94T778VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DCell proliferation and spheroids formation Human diffuse large B-cell lymphoma (DLBLC) SUDHL-10VitroGel Hydrogel MatrixCell viability, growth, drug resistance, proliferation, and cellular matrix interaction Priess human lymphoblastoid cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DEnhance spheroids and cluster formation and promote cell viability. CartilageChondrocytes (bovine) VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell viability and proliferation Chondrocytes (human) VitroGel MSC, VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell viability and proliferation Connective TissueDermal Fibroblasts (human)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell viability and spreading Fibroblasts NIH3T3VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLDirectional cell migration toward gradient and cell spreading dependent on substrata rigidity Foreskin fibroblasts (human)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel YIGSR, VitroGel MMPCell spreading, substrata degradation, and cell invasion Skin fibroblasts (skin) VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel IKVAVCell adhesion Epidermal keratinocytesVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell viability Epithelial CellsMouse ovarian follicle cellsVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel. Human Nthy-ori 3-1 cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DEnhance spheroids and cluster formation and promote cell viability. A549 cellsVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDEnhance cell proliferation and cell matrix interactions. MCF-12AVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDEnhance cell proliferation and cell matrix interactions. Immortalized bronchial epithelial cells HBEC-KRASVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel 3DCell proliferation EyeCorneal endothelial B4G12VitroGel Hydrogel Matrix, VitroGel ORGANOID Kit, VitroGel Angiogenesis AssayVitroGel RGD, Vitrogel Angiogenesis Assay HC kitCell attachment and spreading Retinal ganglion cells (xenopus)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLNeurite outgrowth Immune Cells CD8 T cells VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DEnhance spheroids and cluster formation and promote cell viability. KidneyHuman embryonic kidney HEK293 VitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL3D spheroids formation Madin-Darby Canine KidneyVitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID KitVitroGel RGD, VitroGel MMPEpithelial cysts formation Podocytes (human)VitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLGlomerular capillary formation glomerular endothelial cells (human)VitroGel Hydrogel Matrix, VitroGel HEK293, VitroGel ORGANOID Kit, VitroGel Angiogenesis AssayVitroGel RGD, Vitrogel Angiogenesis Assay HC kitGlomerular capillary formation Liver Hepatocytes (human)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLFilopodia formation and synthesis of albumin and cell attachment Hepatocytes (mouse, rat, swine)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel MMPCell viability, spearding, Albumin secretion LungAlveolar basal epithelial A549VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell attachment Alveolar epithelial RLE-6TNVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell attachment and mesenchymal differentiation Pulmonary fibroblasts LL2 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel IKVAVCell adhesion HFL1 lung fibroblasts CCL153VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell proliferation and spindle morphology Lung cancer associated fibroblasts (human) VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDSubstrata contractility Lung fibroblasts MCR-5VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLNGF-mediated substrata contraction Muscle Myoblasts C2C12 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell proliferation, differentiation, attachment, myofibril formation, myotube formation, and integrin dependent cell adhesion Skeletal myoblasts (mouse)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGDCell attachment, proliferation, and myofibril formation Myoblasts (human)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell adhesion, alignment along fiber, and myotube formation Myoblasts C25Cl48VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLCell proliferation, differentiation and myotube formation Neural Dorsal root ganglion (chick)VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel COLNeurite formation and force dependent neurite outgrowth Neural PC12VitroGel Hydrogel Matrix VitroGel RGD, VitroGel IKVAVNeurite outgrowth Neural stem cell/ progenitor cell (rat)VitroGel STEM, VitroGel Hydrogel MatrixVitroGel RGD, VitroGel IKVAVCell viability, attachment, and differentiation Neural stem cell/ progenitor cell (human)VitroGel STEM, VitroGel Hydrogel MatrixVitroGel RGD, VitroGel IKVAV, VitroGel COLCell viability, attachment, and differentiation Schwann cells (rat)VitroGel Hydrogel MatrixVitroGel RGDCell attachment and migration Neural stem cell/ progenitor cell (mouse)VitroGel STEMVitroGel RGD, VitroGel IKVAVCell adhesion and differentiation Cortical astrocytes (rat)VitroGel Hydrogel MatrixVitroGel RGD, VitroGel IKVAVCell adhesion Spiral ganglion neurons (mouse)VitroGel Hydrogel MatrixVitroGel RGD, VitroGel IKVAVNeurite outgrowth Motor neurons (human)VitroGel Hydrogel MatrixVitroGel RGD, VitroGel COL, VitroGel IKVAVForce dependent neurite outgrowth Forebrain neurons (human)VitroGel Hydrogel MatrixVitroGel RGD, VitroGel COL, VitroGel IKVAVForce dependent neurite outgrowth Cortical neurons (rat)VitroGel Hydrogel MatrixVitroGel RGD, VitroGel COL, VitroGel IKVAVNeuronal viability and neurite outgrowth Dorsal root ganglion (rat)VitroGel Hydrogel MatrixVitroGel RGD, VitroGel COL, VitroGel IKVAVNeurite outgrowth Red Blood CellsRed Blood CellsVitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel 3DEnhance Spheroids and cluster formation and promote cell viability PancreasB-cells MIN6 VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitroGel RGD, VitroGel IKVAVReduced apoptosis and increased insulin release Stem Cells Mesenchymal stem cells (human)VitroGel MSCVitroGel RGD, VitroGel COL, VitroGel IKVAV, VitroGel MMPCell viability, proliferation, differentiation, neuronal differntiation, neurite outgrowth, attachment, spreading, viability, and osteoblast differentiation Mesenchymal stem cells (mouse)VitroGel MSCVitroGel RGD, VitroGel MMPCell spreading and migration Mesenchymal stem cells (rat)VitroGel MSCVitroGel RGDCell adhesion and spreading Embryonic stem cells (mouse)VitroGel STEM, VitroGel ORGANOID KitVitroGel RGD, VitroGel COL, VitroGel YIGSREndothelial cell differentiation, neuronal differentiation, and neurite outgrowth Induced pluripotent stem cells (human)VitroGel STEM, VitroGel ORGANOID KitVitroGel RGD, VitroGel YIGSR, VitroGel IKVAVCell viability Human stem cells from apical papilla SCAPVitroGel STEMVitroGel RGDCell viability Hematopoietic Stem CellsVitroGel STEMCell viability Adipose derived stem cells (human)VitroGel MSCVitroGel RGD, VitroGel 3D, VitroGel IKVAVCell viability, cell attachment Vascular/cardiac Umbilical vein endothelial cells Vitrogel Angiogenesis Assay , VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitrogel Angiogenesis Assay HC kitCell attachment, proliferation, migration, angiogenesis, gene expression changes, migratory cell infiltration, cell survival, and VEGF dependent migration Neonatal cardiac (rat) Vitrogel Angiogenesis Assay, VitroGel Hydrogel Matrix, VitroGel ORGANOID KitVitrogel Angiogenesis Assay HC kitCell attachment, tissue regeneration, and attachment similar to laminin Aortic smooth muscle cells VitroGel Hydrogel MatrixVitrogel Angiogenesis Assay HC kitCell attachment Endothelial (human)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell differentiation EndotheliocytesVitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell migration Microvascular endothelial cells (human)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCell mobility Aortic endothelial cells (bovine)VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitForce dependent cell spreading Capillary endothelial cells (bovine) VitroGel Angiogenesis Assay Vitrogel Angiogenesis Assay HC kitCapillary like network formation Data Figure 1.  Rheological properties of VitroGel® RGD with DMEM medium. A) – C) The gel formation curve after mixing with DMEM (A), DMEM/F-12 (B), and RPMI (C) media. VitroGel RGD was diluted at 1:0,1:1, 1:2 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with media at 4:1 (v/v) ratio; D) – F) The gel strength after 24 hrs incubation in DMEM (D), DMEM/F-12 (E), and RPMI (F) media. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa) Figure 2. 3D culture of OP9 cells in VitroGel® RGD. Hydrogel was prepared at 1:3 dilution with VitroGel® Dilution Solution (Type 1). The images were taken on days 2 and 7.  VitroGel® RGD shows support for OP9 cell proliferation and cell-cell communication. The stronger cell-matrix interactions help the cells to form the cell-networking structure. Figure 3. 3D view of OP9 cells growth in VitroGel® RGD. Cell networking structure formed in VitroGel® RGD. Figure 4. 3D culture of U87-MG cells in VitroGel® RGD. Cells can grow in 3D hydrogel at 1:1 and 1:3 dilution of VitroGel® RGD. U87-MG cells exhibit a cell networking structure and morphology in VitroGel® RGD, indicating cell-cell and cell-matrix interactions. References/Publications Qiao, X., Zhang, X., Tian, T., Li, Y., Qiao, S., He, F., & Xing, L. (2026). PTBP1 Facilitates Acute Myeloid Leukemia Cell Migration, Invasion, and Expression of EMT Markers by Regulating WNK1. Frontiers in Bioscience-Landmark, 31(3). https://doi.org/10.31083/fbl47982 Sutterby, E. (2025). A Customisable LED System for Standardised Photobiomodulation Research Using Advanced Skin Modelling. RMIT. https://doi.org/10.25439/rmt.30185068 Acimovic, I., Chochola, V., Herrera, J. L., Hampl, A., & Jaros, J. (2025). 3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors. 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