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RocketCell™ Organoid Xeno-Free Essential-Core Medium One Core Medium. Endless Organoid Possibilities. Unified Core System Replaces the need for separate supplementation, providing a complete nutritional foundation in one simple kit. Plug-and-Play Flexibility Serves as a versatile base for multiple organoid lineages and differentiation stages; just add the specific growth factors required for your specific model. 100% Xeno-Free & Defined Ensures a controlled, animal-origin-free environment, minimizing batch-to-batch variability and regulatory hurdles. Broad Matrix Compatibility Validated for use with both synthetic and animal-derived hydrogels, enabling seamless integration into existing organoid platforms. Enhanced Viability & Recovery Supports robust initial survival and maintains high viability through repeated passaging and long-term culture. Maximum Efficiency Drastically reduces media preparation time and calculation errors, allowing researchers to focus on data rather than bench work. The RocketCell™ Organoid Xeno-Free Essential-Core Medium Kit (TheWellBioscience, Cat# RC04-OCM) is a standardized, chemically defined foundation medium designed to simplify and unify organoid culture workflows. This ready-to-use kit serves as a fundamental medium that provides all essential nutrients and is flexible, allowing direct mixing with growth factors and signaling molecules to support various types of organoid development. It eliminates the need for conditioned media preparation or complex multi-component media and supplement mixing. This advanced Essential-Core formulation replaces multiple supplementation steps (such as N2/B27) with a single, defined system. Researchers can generate customized, organoid-specific media simply by adding the desired growth factors and signaling molecules. Developed to support long-term expansion, high post-passaging viability, and reproducible outcomes, RocketCell™ Essential-Core Medium establishes a flexible, xeno-free platform for diverse organoid systems in both synthetic and animal-derived hydrogels. This medium supports organoids from different sources and can also serve as a flexible base for formulating differentiation media tailored to the developmental stages of iPSC-derived organoids. By providing a unified and consistent core formulation, this kit enables the establishment of standardized, reproducible workflows for organoid culture across research, translational, and industrial settings. Specifications UseFor Organoid Cultures Cell HarvestingVitroGel® Organoid Recovery Solution 5-15 min cell recovery Shipping and StorageBasal Medium: Ships with ice pack. Store at 2-8°C. Supplement: Ships with dry ice. Store at -20°C. Complementary Product CytoGrow™ Growth Factors Challenges We Solve Key Challenges in Organoid Culture Complexity and Variability Preparing a conditional medium or mixing multiple supplements leads to batch inconsistency. Laborious Preparation Tedious calculations and labor-heavy media prep waste valuable research time. Media Fragmentation Buying specialized kits for every organoid type creates rigid, non-transferable workflows. High Costs and Waste Prohibitive kit prices and expired unused reagents from specialized media inflate budgets. RocketCell™ Organoid Essential-Core Advantages Standardized Reproducibility Replaces complex preparation procedures with a pre-optimized core for guaranteed consistency. Time Efficiency A ready-to-use system turns hours of prep work into just a few minutes. Universal Foundation One flexible core for all organoid types. Simple add the specific growth factors your need. Economical Scaling A single, versatile core reduces inventory costs and eliminates the waste of expired, niche media. Growth Factors by Organoid Type Perfect Match with CytoGrow™ Premium-Grade Growth Factors for Organoid Culture CytoGrow™ is TheWell Bioscience’s portfolio of premium-grade recombinant proteins that pair seamlessly with RocketCell™ Organoid Xeno-Free Essential Core Medium to enhance cellular activity and drive superior performance. Organoid TypesKey Growth Factors BrainBDNF (CG002),EGF (CG014), FGF-2 (CG003), FGF-8b (CG020), GDNF (CG092), NT-3 (CG105), SHH, TGF-β1(CG069) BreastEGF (CG014), FGF-2 (CG003), FGF-7 (CG019), FGF-10 (CG022), NRG1-β1 (CG106), Noggin (CG059), R-Spondin1 (CG067), R-Spondin 3, Wnt-3a (CG084) GastricActivin-A (CG001), BMP-4, EGF (CG014), FGF-4 (CG018), FGF-10 (CG022), Gastrin, Noggin (CG059), R-Spondin1 (CG067), Wnt-3a (CG084) Inner EarBMP-4, FGF-2 (CG003), FGF3, FGF-10 (CG022) IntestinalActivin-A (CG001), BMP-4, EGF (CG014), FGF-4 (CG018), Noggin (CG059), R-Spondin1 (CG067), Wnt-3a (CG084) KidneyActivin-A (CG001), BMP-2 (CG006), BMP-4, BMP-7 (CG007), FGF-2 (CG003), FGF-7 (CG019), FGF-9 (CG021), GDNF (CG092), R-Spondin1 (CG067) LiverActivin-A (CG001), BMP-4, BMP-7 (CG007), EGF (CG014), FGF-2 (CG003), FGF-7 (CG019), FGF-10 (CG022), FGF-19 (CG119), Gastrin HGF (CG032), IGF-1 (CG120), Noggin (CG059), OSM (CG061), R-Spondin1 (CG067), VEGF165 (CG080), Wnt-3a (CG084) LungActivin-A (CG001), FGF-4 (CG018), FGF-7 (CG019), FGF-10 (CG022), Noggin (CG059), R-Spondin1 (CG067), SHH, Wnt-3a (CG084) ProstateEGF (CG014), FGF-10 (CG022), Noggin (CG059), R-Spondin1 (CG067), Wnt-3a (CG084) RetinalFGF-2 (CG003), IGF-1 (CG120), Noggin (CG059), SHH, Wnt-3a (CG084) Recommended Growth Factors per Organoid Type Protocols and Resources RocketCell™ Organoid Xeno-Free Essential-Core Medium – Instruction Manual Product Documentation RocketCell™ Organoid Xeno-Free Essential-Core Medium – Sale Sheet Material Safety Data Sheet Frequently Asked Questions Data and References Case 1: Use of RocketCell™ Organoid Xeno-Free Essential-Core Medium in Mouse Intestinal Organoid Generation in Xeno-Free VitroGel® ORGANOID hydrogel Figure 1: Use of RocketCell™ Organoid Xeno-Free Essential-Core Medium in Generating Mouse Intestinal Organoid. RocketCell™ Organoid Xeno-Free Essential-Core Medium was prepared according to the manufacturer’s instructions. The medium was then supplemented with intestinal organoid–specific growth factors (e.g., Wnt pathway agonists, EGF, Noggin, and R-spondin) as required. The resulting complete medium was used in combination with VitroGel® ORGANOID hydrogel to support intestinal organoid formation, growth, and maintenance. (A) Organoids (VitroGel® passaged) fragments cultured in VitroGel® ORGANOID: Day 0. (B) Mouse intestinal organoids were maintained in intestinal organoid media prepared using RocketCell™ Organoid Xeno-Free Essential-Core Medium. Organoid growth after 12 days. (C) Normalized mouse intestinal organoid area from day 0 to day 12: 3 wells. Note: Only well 1 set of results is included. Case 2: Intestinal Organoid Growth Comparison: RocketCell™ Organoid Xeno-Free Essential-Core Medium vs Commercial Intestinal Organoid media | Animal-based ECM Figure 2: Intestinal Organoid Growth Comparison: RocketCell™ Organoid Xeno-Free Essential-Core Medium vs. Commercial Intestinal Organoid Media | Animal-Based ECM. RocketCell™ Organoid Xeno-Free Essential-Core Medium and commercial intestinal organoid media were prepared according to the manufacturer’s instructions. The 1X RocketCell™ Organoid Xeno-Free Essential-Core Basal Medium was then supplemented with intestinal organoid-specific growth factors (e.g., Wnt pathway agonists, EGF, Noggin, and R-Spondin1). Organoids were cultured in an animal-derived gel (Matrigel) as domes, and growth was compared between 2 media types. (A&B) Organoid fragments cultured in media prepared using RocketCell™ Organoid Xeno-Free Essential-Core Medium and commercial intestinal organoid media: Day 0. (C&D) Intestinal organoid growth after 5 days. (E) Normalized mouse intestinal organoid area from day 0 to day 5. Case 3: iPSC-derived Intestinal Organoids Generated using RocketCell™ Organoid Xeno-Free Essential-Core Medium and Xeno-Free VitroGel® ORGANOID Hydrogel C. Figure 3: Use of RocketCell™ Organoid Xeno-Free Essential-Core Medium in Generating iPSC Derived Intestinal Organoid. RocketCell™ Organoid Xeno-Free Essential-Core Medium was prepared according to the manufacturer’s instructions. This medium then serves as a flexible base for formulating differentiation media tailored to each developmental stage of iPSC-derived intestinal organoid differentiation. Mature organoids were cultured in VitroGel® ORGANOID hydrogel and maintained. (A) iPSC-derived organoids (VitroGel passaged) fragments cultured in VitroGel® ORGANOID: Day 0. (B) Intestinal organoids were maintained in media prepared using RocketCell™ Organoid Xeno-Free Essential- Core Medium. Organoid growth after 18 days. (C) Normalized intestinal organoid area from day 0 to day 10. (D) A movie clip on organoid growth. Case 4: Immunofluorescence staining: iPSC-derived Intestinal Organoids Cultured Using RocketCell™ Organoid Xeno-Free Essential-Core Medium in Xeno-Free VitroGel® ORGANOID Hydrogel Figure 4: Use of RocketCell™ Organoid Xeno-Free Essential-Core Medium in Generating iPSC-Derived Intestinal Organoid. RocketCell™ Organoid Xeno-Free Essential-Core Medium with VitroGel® hydrogels, CytoGrow™ growth factors were used to generate iPSC-derived intestinal organoids. Representative image showing iPSC-derived intestinal organoids cultured in VitroGel® ORGANOID 5. iPSC cells were 1st cultured in a medium supplemented with RocketCell™ Organoid Xeno-Free Essential-Core with VitroGel® STEM to generate spheroid cells. These spheroids were then differentiated into intestinal organoids using CytoGrow™ growth factors & immunofluorescence staining was performed. Images (A) & (C) represent the center of iPSC-derived intestinal organoid & 3D structure, respectively. Yellow fluorescence: Phalloidin, Magenta: Villin protein, Blue staining (DAPI) represents cell nuclei. (B) Max projected an image of the same intestinal organoid. (D) A movie clip of an intestinal organoid stained with Cyto3D® Live-Dead Assay reagent Case 5: iPSC-derived Liver Organoid Using RocketCell™ Organoid Xeno-Free Essential-Core Medium and Xeno-Free VitroGel® ORGANOID Hydrogel Figure 5: Use of RocketCell™ Organoid Xeno-Free Essential-Core Medium in Generating iPSC-derived Liver Organoids. RocketCell™ Organoid Xeno-Free Essential-Core Medium was prepared and supplemented with organoid–specific growth factors at different development stages until mature liver organoids are generated. Mature organoidswere cultured in VitroGel® ORGANOID hydrogel and maintained. (A) iPSC-derived spheroids generated in RocketCell™ Organoid Xeno-Free Essential-Core Medium. (B) Definitive endoderm differentiated spheroids. (C) Hepatic endoderm differentiated spheroids. (D) Early hepatic maturation. (E) Liver organoids with hepatic maturation. (F) Mature differentiated liver organoids generated in media supplemented with RocketCell™ Organoid Xeno-Free Essential-Core Medium and CytoGrow™ growth factors: Day 30. Case 6: Live-Dead Assay: Liver Organoids Culture Using RocketCell™ Organoid Xeno-Free Essential-Core Medium Figure 6: Live–dead Assay in Liver Organoids cultured using RocketCell™ Organoid Xeno-Free Essential-Core Medium & CytoGrow™ Growth Factors. Organoids were cultured in RocketCell™ Organoid Xeno-Free Essential-Core supplemented medium, and liver organoids with hepatic maturation were then stained with Cyto3D® Live-Dead Assay Kit (2 μL of reagent for every 100 μL media). After 15-minute incubation at 37˚C, organoids were observed under a fluorescence microscope. (A) A bright-field image of a liver organoid hepatic maturation. Images show live cells (B: Green) and dead cells (C: Red) in the liver organoid. (D) Merged images. (E) A liver organoid stained with Cyto3D® Live-Dead Assay Kit & red indicates dead cells; Green indicates live cells. (F) A movie clip of a liver organoid stained with Cyto3D® Live-Dead Assay reagent. Webinars Streamlining Organoid Culture with a Universal RocketCell™ Organoid Xeno-Free Essential-Core Medium Enhance your organoid culture with these products: Hydrogels - Ready-To-Use VitroGel® ORGANOID ready-to-use, xeno-free (animal origin-free) hydrogel system for organoid culture New 3D Culture Imaging Plates VitroPrime™ 3D Culture and Imaging Plate A premium cover-glass bottom plate for zero disruption 3D cell culture workflow: From cell seeding to high-resolution imaging. CytoGrow™ - Recombinant Proteins EGF (Human), Recombinant Protein, CHO, Tag Free Premium CytoGrow™ growth factor suitable for cell culture supplementation, wound healing research, epithelial biology, and cancer signaling studies. Accessory Proteins R-Spondin1 (Human), Recombinant Protein, CHO, Tag Free Premium CytoGrow™ growth factor suitable for stem cell research, tissue regeneration, developmental biology, and cancer studies. 3D Culture Vessels VitroPrime™ Spread-Attach Plate Unique surface treated for superior hydrogel spreading, adherence, and uniform surface. Cell Harvesting Solution VitroGel® Organoid Recovery Solution Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes. Downstream Cell Analysis Cyto3D® Live-Dead Assay Kit Fast (15 min), versatile, live/dead cell viability analysis for 3D and 2D cell culture. IN STOCK
Ready-To-Use Hydrogels
