RocketCell™ NSC Xeno-Free Complete Growth Kit

RocketCell™ NSC Xeno-Free Complete Growth Kit All-in-one, ready-to-use kit for 3D and 2D neural stem cell culture Ready-to-use, All-in-one Kit 100% synthetic kit with optimal combination of matrix and medium for 2D and 3D NSC culture and maintenance. Supports 3D NSC Network Formation Promotes 3D neurite and axonal outgrowth for 3D networking formation, ideal for advanced studies. Enhanced Cell Adhesion with Advanced Hydrogel 4X more usage than animal-based ECM for a robust, simple coating for cell adhesion Long-Term Culture and Maintenance Culture NSCs for over 4 weeks without compromising their multipotency.  Simple 3D and 2D Workflows at Room Temperature Room temperature operation with simple mixing or coating steps; supports lab automation.  Easy Cell Harvesting Simple and efficient cell harvesting with VitroGel® Organoid Recovery Solution The RocketCell™ NSC Xeno-Free Complete Growth Kit is an all-in-one, ready-to-use kit designed to support the expansion and maintenance of neural stem cells (NSCs) in both 3D and 2D formats. This kit offers a fully defined, optimized combination of a synthetic hydrogel and a xeno-free complete medium, eliminating variability and ensuring consistent, reproducible results. The RocketCell™ NSC Xeno-Free Complete Growth Kit provides a robust, scalable platform for long-term culture and large-scale expansion of undifferentiated NSCs in both 3D and 2D formats—while maintaining their multipotency and functional integrity. Designed for convenience and performance, this ready-to-use system operates at room temperature, integrates seamlessly with high-resolution imaging and high-throughput screening workflows for advanced neuroscience and regenerative medicine research. The all-in-one kit includes: Kit Contents: 1 x 2 mL VitroGel® NEURON, the first-of-its-kind synthetic biofunctional hydrogel for NSC and neuronal cultures​ 1 x 500 mL, RocketCell™ NSC Xeno-Free Basal Medium​ 1 x 10 mL, RocketCell™ NSC Xeno-Free Supplement (50X)​ 1 x 10 µg CytoGrow™ Recombinant EGF (Human), CHO, Tag Free 1 x 10 µg, CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free We offer variations of the NSC Kit to fit your workflow: RocketCell™ 3D NSC Xeno-Free Complete Growth Kit↗ An all-in-one, ready-to-use kit for 3D neural stem cell culture. RocketCell™ NSC Xeno-Free Growth Medium↗  Cell culture medium for NSC culture Specifications Use3D and 2D proliferation and maintenance of neural stem cells FormulationXeno-Free, biofunctional hydrogel and complete growth media Injectability Injectable hydrogel for in vivo studies and lab automation​ Operation Ready-to-use at room temperature No. of Use 2 mL kit, we recommend doing a ratio of 1:100-1:200 dilutions. 6-well: 30-60 plates 24-well plate: 25-50 plates Cell Harvesting VitroGel® Organoid Recovery Solution​ 5-15 min cell recovery Shipping and StorageHydrogel: Ships at ambient temperature. Store at 2-8°C. Basal Medium: Ships with ice pack. Store at 2-8°C. Supplement Dry Ice shipping. Store at -20- -80°C. Growth Factors: Ships with Ice pack/Dry ice. Store at -20- -80°C. Challenges We Solve Common Challenges in NSC Culture Lack of a complete xeno-free platform for 3D and 2D NSC Culture Spontaneous differentiation in animal-based ECMs Poor cell matrix and cell-cell interaction for 3D neurite axonal outgrowth Lack of an optimal combination of matrix and xeno-free complete medium for NSC culture Difficult to perform long-term NSC Culture How RocketCell™ NSC Xeno-Free Complete Growth Kit Solves The Issues​ Supports 3D NSC Culture Enables both single cells and 3D spheroids to grow within VitroGel® NEURON hydrogel. Improved Cell Attachment for 2D NSC Culture Use 4X less concentration than animal-based ECM to support a fast and reliable coating method at room temperature. Enhanced 3D Cell Connectivuty and Network Formation Robust network formation and axonal projections mimicking the in vivo environment. Complete all-in-one package Optimal xeno-free combination of hydrogel matrix and culture medium for robust and reliable results every time. Long-Term Culture and Neglibile Spontaneous Differentiation Over 4 weeks in culture without compromising multipotency in both 2D and 3D Protocols and Resources   RocketCell™ NSC Xeno-Free Growth Complete Kit – Protocol  VitroGel® NEURON Protocol Product Documentation RocketCell™ NSC Xeno-Free Complete Growth Kit – Sale Sheet Product Data Sheet Material Safety Data Sheet (MSDS) Frequently Asked Questions  Data and References 2D THIN COATING METHOD  2D: CASE 1 2D NSC Maintenance (1:200 coating on VitroPrime™ Spread-Attach Plate)​ A   B Figure 1. VitroGel® NEURON 2D Thin Coating for Growth of iPSC-Derived NSCs and Confluence Analysis on VitroPrime™ Spread-Attach Plates.​ A. iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with either VitroGel® NEURON (1:200) or Geltrex®, and cultured for 6 days. Images were captured using the Incucyte® S3 system. B. NSC growth was quantified by percent confluence over time, with images collected every 8 hours and analyzed relative to the initial timepoint (T = 0 4 hrs).​ 2D: CASE 2 2D NSC Maintenance and Characterization (1:200 coating on VitroPrime™ Spread-Attach Plate)​ Figure 2. NSC Characterization after Subculturing with the RocketCell™ NSC Xeno-Free Complete Growth Kit. iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with VitroGel® NEURON (1:200). Immunofluorescence staining depicts the presence of cells positive for Nestin (A, green) and Sox2 (B, red), which are NSC markers. The nuclei were stained with DAPI (A, blue) or Hoescht (B, blue). 3D NEURON CULTURE  3D: CASE 1 3D NSC Culture with VitroGel® NEURON as Single Cell Encapsulation​ Figure 3. 3D Encapsulation Culture of NSC cells as a Single Cell Suspension in VitroGel® NEURON​ VitroGel® NEURON Hydrogel and RocketCell™ NSC Xeno-Free Media support 3D NSC cultures and neurite outgrowth. (A) 3D NSC cultures were established by preparing a single-cell NSC suspension with RocketCell™ NSC Xeno-Free Medium and mixing with VitroGel® NEURON for 6 days. (B) Immunofluorescence staining was performed to evaluate the presence of beta-III-tubulin, a neuron maturation marker. The images were obtained with the Keyence BZ-X microscope at 10 and 20X magnifications. 3D: CASE 2 3D Characterization of Differentiated NSC Spheroids Figure 4. RocketCell™ NSC Xeno-Free Complete Growth Kit supports the expansion of NSCs for differentiation with the RocketCell™ NSC-Neuron Differentiation Kit​. iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Complete Growth Kit. The cells were then differentiated into neurons with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The samples were fixed and stained to evaluate the expression of (A) Microtubule-associated protein 2 (MAP2; green), (B) Neurofilament-H (red), and (C) Synaptophysin (purple). The nuclei were stained with Hoescht dye. 3D: CASE 3 3D Development of Dopaminergic Neurons from NSC spheroids​ A B Figure 5. VitroGel® NEURON hydrogel supports the 3D development of dopaminergic neurons.​ iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit for 6 passages and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Complete Growth Kit (A, Day 0). To induce NSC differentiation into dopaminergic neurons, the media were replaced with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The cells were exposed to the medium for 16 days (A, Day 16). The samples were fixed and stained with beta-III-tubulin and DAPI (B). It is apparent that most spheroids are neurons, and axonal connections are clearly visible at higher magnification. ​ 3D: CASE 4 3D Encapsulation Culture of NSCs Figure 6.  3D encapsulation culture of NSCs in VitroGel® NEURON hydrogel as NSC spheroids with zonal projections. iPSCs were cultured and expanded using the RocketCell™ 3D iPSC Xeno-free Complete Growth kit. The cells were harvested from the hydrogel with the VitroGel® Organoid Recovery Solution and embedded in VitroGel® NEURON to induce iPSC differentiation into NSCs with CytoGrow™ Growth factors. The NSCs were differentiated into dopaminergic neurons with the RocketCell™ NSC Neuron differentiation kit. Resources Poster Development of In Vitro Developing a Biofunctional Xeno-free Hydrogel Platform for Long-term 3D Neuronal Differentiation and Maintenance Poster Development of In Vitro The Growth and Neuronal Differentiation of Human Induced Pluripotent Stem Cells in Xeno-Free Functional Synthetic Hydrogel​ Enhance your neuron cell culture with these products: New Cell Culture Medium (Xeno-Free) RocketCell™ 3D NSC Xeno-Free Complete Growth Kit All-in-one, ready-to-use kit for 3D neural stem cell culture. New Cell Culture Medium (Xeno-Free) RocketCell™ NSC Xeno-Free Growth Medium Xeno-Free complete medium for 3D and 2D neural stem cell culture CytoGrow™ - Recombinant Proteins bFGF/FGF-2 (Human), Recombinant Protein, E. coli, Tag Free Premium CytoGrow™ growth factor suitable for stem cell culture, angiogenesis research, wound healing studies, and regenerative medicine. CytoGrow™ - Recombinant Proteins EGF (Human), Recombinant Protein, CHO, Tag Free Premium CytoGrow™ growth factor suitable for cell culture supplementation, wound healing research, epithelial biology, and cancer signaling studies. 3D Culture Vessels VitroPrime™ Spread-Attach Plate Unique surface treated for superior hydrogel spreading, adherence, and uniform surface. Cell Harvesting Solution VitroGel® Organoid Recovery Solution Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes. Downstream Cell Analysis Cyto3D® Live-Dead Assay Kit Fast (15 min), versatile, live/dead cell viability analysis for 3D and 2D cell culture.  IN STOCK