RocketCell™ NSC Xeno-Free Growth Medium Xeno-Free complete medium for 3D and 2D neural stem cell culture The RocketCell™ NSC Xeno-Free Growth Medium is designed to support the expansion and maintenance of neural stem cells (NSCs) in both 3D and 2D formats. Contents: 1 x 500 mL, RocketCell™ NSC Xeno-Free Basal Medium 1 x 10 mL, RocketCell™ NSC Xeno-Free Supplement (50X) 1 x 10 µg CytoGrow™ Recombinant EGF (Human), CHO, Tag Free 1 x 10 µg, CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free We offer variations of the NSC Kit to fit your workflow: RocketCell™ 3D NSC Xeno-Free Complete Growth Kit↗ An all-in-one, ready-to-use kit for 3D neural stem cell culture. RocketCell™ NSC Xeno-Free Complete Growth Kit↗ An all-in-one, ready-to-use kit for 2D neural stem cell culture. Specifications Use3D and 2D proliferation and maintenance of neural stem cells Shipping and StorageBasal Medium: Ships with ice pack. Store at 2-8°C. Supplement Dry Ice shipping. Store at -20- -80°C. Growth Factors: Ships with Ice pack/Dry ice. Store at -20- -80°C. Protocols and Resources RocketCell™ NSC Xeno-Free Growth Medium – Protocol VitroGel® NEURON Protocol Product Documentation RocketCell™ NSC Xeno-Free Growth Medium – Sale Sheet Product Data Sheet Material Safety Data Sheet (MSDS) Frequently Asked Questions Data and References 2D: CASE 1 2D NSC Maintenance and Characterization (1:200 coating on VitroPrime™ Spread-Attach Plate) Figure 1. NSC Characterization after Subculturing with the RocketCell™ NSC Xeno-Free Growth Medium. iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with VitroGel® NEURON (1:200). Immunofluorescence staining depicts the presence of cells positive for Nestin (A, green) and Sox2 (B, red), which are NSC markers. The nuclei were stained with DAPI (A, blue) or Hoescht (B, blue). 3D: CASE 1 3D Characterization of Differentiated NSC Spheroids Figure 2. RocketCell™ NSC Xeno-Free Growth Medium supports the expansion of NSC spheroids for differentiation. iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Growth Medium. The cells were then differentiated into neurons with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The samples were fixed and stained to evaluate the expression of (A) Microtubule-associated protein 2 (MAP2; green), (B) Neurofilament-H (red), and (C) Synaptophysin (purple). The nuclei were stained with Hoescht dye. Resources Poster Development of In Vitro Developing a Biofunctional Xeno-free Hydrogel Platform for Long-term 3D Neuronal Differentiation and Maintenance Poster The Growth and Neuronal Differentiation of Human Induced Pluripotent Stem Cells in Xeno-Free Functional Synthetic Hydrogel Enhance your neuron cell culture with these products: New Cell Culture Medium (Xeno-Free) RocketCell™ 3D NSC Xeno-Free Complete Growth Kit All-in-one, ready-to-use kit for 3D neural stem cell culture. New Cell Culture Medium (Xeno-Free) RocketCell™ NSC Xeno-Free Complete Growth Kit All-in-one, ready-to-use kit for 3D and 2D neural stem cell culture. CytoGrow™ - Recombinant Proteins EGF (Human), Recombinant Protein, CHO, Tag Free Premium CytoGrow™ growth factor suitable for cell culture supplementation, wound healing research, epithelial biology, and cancer signaling studies. 3D Culture Vessels VitroPrime™ Spread-Attach Plate Unique surface treated for superior hydrogel spreading, adherence, and uniform surface. Cell Harvesting Solution VitroGel® Organoid Recovery Solution Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes. Downstream Cell Analysis Cyto3D® Live-Dead Assay Kit Fast (15 min), versatile, live/dead cell viability analysis for 3D and 2D cell culture. IN STOCK
