RocketCell™ Intestinal Organoid Xeno-Free Growth Kit All-in-one, xeno-free kit for growth expansion and passaging of intestinal organoids. RocketCell™ Intestinal Organoid Xeno-Free Growth Kit is a ready-to-use, chemically defined solution engineered to support every stage of human intestinal organoid workflows — from initial seeding and organoid formation through maturation and downstream applications. The kit pairs a tunable, xeno-free hydrogel matrix (VitroGel® ORGANOID-5) with three purpose-formulated RocketCell™ IO XF media (IO XF 1, IO XF 2, IO XF 3) that guide cells through establishment, expansion, and differentiation. The result is consistent organoid morphology, improved reproducibility across batches, and elimination of animal-derived components for translational studies and regulatory-sensitive applications. Key Features & Benefits Xeno-free and chemically defined: removes animal components to reduce variability and improve translational relevance. Stage-specific media series: IO XF 1 for seeding and organoid initiation, IO XF 2 for expansion, IO XF 3 for maturation and differentiation. VitroGel® ORGANOID-5 hydrogel: tunable mechanical properties and defined composition that mimic intestinal ECM without animal origin. Simple, streamlined workflow: pre-optimized formulations reduce protocol development time and accelerate experimental throughput. Scalable and automation friendly: Compatible with multiwell formats and plate-based assays for high-content screening. Reproducible performance: Batch control and defined reagents support consistent results across runs and users. Specifications Kit Contents• VitroGel® ORGANOID-5 (10 mL) • RocketCellTM IO XF Medium 1 (5 mL) • RocketCellTM IO XF Medium 2 (20 mL) • RocketCellTM IO XF Medium 3 (100 mL) HydrogelXeno-Free Formulation, Room-temperature Stable ShippingShip at ambient temperature • VitroGel® ORGANOID-5 (10 mL) Requires dry ice shipping • RocketCellTM IO XF Medium 1 (5 mL) • RocketCellTM IO XF Medium 2 (20 mL) • RocketCellTM IO XF Medium 3 (100 mL) StorageStore at 2-8°C • VitroGel® ORGANOID-5 (10 mL) Store at -20°C • RocketCellTM IO XF Medium 1 (5 mL) • RocketCellTM IO XF Medium 2 (20 mL) • RocketCellTM IO XF Medium 3 (100 mL) Protocols and Resources RocketCell™ Intestinal Organoid Xeno-Free Growth Kit – Protocol Product Documentation RocketCell™ Intestinal Organoid Xeno-Free Growth Kit – Sale Sheet Product Data Sheet Material Safety Data Sheet (MSDS) Frequently Asked Questions Data and References Figure 1. Use of VitroGel® hydrogels to generate iPSC-derived intestinal organoids. Representative image showing iPSC-derived intestinal organoids cultured in VitroGel® ORGANOID. iPSC cells were cultured in VitroGel® STEM to generate spheroid cells. The spheroids were then differentiated into intestinal organoids using CytoGrow™ growth factors. (A)Yellow fluorescence: Phalloidin, (B) Magenta: Villin protein, (C) Blue staining (DAPI) represents cell nuclei. (D) Merged image of the iPSC-derived intestinal organoid Blog News Inside-Out or Outside-In? How Organoid Polarity Shapes Nutrition and Drug Discovery Research
